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KMID : 0375319940160020257
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Journal of Clinical Pathology and Quality Control
1994 Volume.16 No. 2 p.257 ~ p.262
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Comparison of the BACTO STAOPH LATEX TEST, Slide, and Tube Coagulase Tests for the Identification of Staphylococcus aureus
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조기섭
오재혁/김대근/김정욱/이웅수/최태열
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Abstract
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Background:
The coagulase test is the most widely used procedure for identification of Staphylococcus aureus. The purpose of this investigation is to evaluate a commercial product, the BACTO STAPH LATEX TEST(Difco Lab., USA), and the slide and tube
coagulase
tests used for the identification of S. aureus isolated from clinical sources.
Methods:
A total of 110 clinically isolated staphylococci were identified with the API STAPH(Biomerieux, France), catalase, mannitol fermentation, and DNase tests. The BACTO LATEX TESTS were compared with the slide coagulase(SC) and tube coagulase (TC)
tests
at room temperature (22 to 25℃) and at 37℃. The results of the TC test were read after 4 hours and then following overnight incubation(18-24 hours).
Results:
The sensitivity and specficity of the SC test were 98% and 97%, respectively. There were one false negative and two false positive results. The sensitivity and specificity of tube coagulase test after 4 hours were 80% and 100%, respectively.
And the
results of TC test after overnight incubation were each 100%, respectively. The results of the TC test at room temperature and 37℃ were the same. The BACTO STAPH LATEX TEST correctly identified 41(98%) of the S. aureus and 68(100%) of the non-S.
aureus
isolates, respectively.
Conclusions:
Based on these results, we recommend that the BACTO STAPH LATEX TEST and SC test be used fro screening of coagulase test, and the TC test as screening and confirmatory test should be interpretated after overnight incubation.
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